產(chǎn)品展示首頁 > USCN & cloud-clone 試劑盒 & 抗體 & 蛋白 > cloud-clone 試劑盒、抗體、蛋白 > Cytochrome C Oxidase Subunit II (COX2)
細(xì)胞色素C氧化酶亞基Ⅱ(COX2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
貨 號:
SED284Mu-96T
規(guī) 格:
96T
品 牌:
cloud-clone
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英文名稱: | ELISA Kit for Cytochrome C Oxidase Subunit II (COX2) |
基因別稱: | MTCO2; MT-CO2; COII; COXII; Mitochondrially Encoded Cytochrome C Oxidase II; Cytochrome c oxidase polypeptide II |
實驗步驟: | 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC; 8. Add 50µL Stop Solution. Read at 450nm immediately. |
檢測范圍: | 0.312-20ng/mL |
最低檢測限: | The minimum detectable dose of this kit is typically less than 0.114ng/mL |
適應(yīng)生物: | Mus musculus (Mouse) |
樣本: | tissue homogenates, cell lysates and other biological fluids |
實驗時長: | 3h |
種類: | 參考價格:3024/48T ; 4320/96T ; 19440/96T*5 ; 36720/96T*10 ; 302400/96T*100 ; |
質(zhì)量控制: | ISO13485:2003 EN ISO13485:2012/ISO9001:2008 |
產(chǎn)品應(yīng)用: | ELISA , 酶聯(lián)免疫 |
現(xiàn)貨: | 5-7個工作日 |
運輸條件: | 藍(lán)冰運輸 |
保存條件: | 4度和-20度分開保存,避免反復(fù)凍融 |
有效期: | 6個月 |
限制: | 僅供科研使用 |
標(biāo)本量: | 詳見說明書 |
需自備的設(shè)備及試劑: | 1、450±10nm濾光片的酶標(biāo)儀(建議儀器使用前提前預(yù)熱); 2、單道或多道微量加液器及吸頭; 3、稀釋樣品的EP管; 4、蒸餾水或去離子水; 5、吸水紙; 6、盛放洗液的容器 |
Research_Areas: | Enzyme & Kinase; |
Emit: | Cytochrome C Oxidase Subunit II |