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產品展示首頁 > USCN & cloud-clone 試劑盒 & 抗體 & 蛋白 > cloud-clone 試劑盒、抗體、蛋白 > ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2)
氫離子轉運ATP酶溶酶體輔助蛋白2(ATP6AP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
貨    號:
SEG393Hu-96T
規(guī)    格:
96T
品    牌:
cloud-clone
英文名稱: ELISA Kit for ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2)
基因別稱: ATP6IP2; CAPER; ELDF10; XMRE; Renin/prorenin receptor; Embryonic liver differentiation factor 10; Vacuolar ATP synthase membrane sector-associated protein M8-9
實驗步驟: 1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
檢測范圍: 0.312-20ng/mL
最低檢測限: The minimum detectable dose of this kit is typically less than 0.115ng/mL
適應生物: Homo sapiens (Human)
樣本: tissue homogenates, cell lysates and other biological fluids
實驗時長: 3h
種類: 參考價格:2940/48T ; 4200/96T ; 18900/96T*5 ; 35700/96T*10 ; 294000/96T*100 ;
質量控制: ISO13485:2003 EN ISO13485:2012/ISO9001:2008
產品應用: ELISA , 酶聯(lián)免疫
現(xiàn)貨: 5-7個工作日
運輸條件: 藍冰運輸
保存條件: 4度和-20度分開保存,避免反復凍融
有效期: 6個月
限制: 僅供科研使用
標本量: 詳見說明書
需自備的設備及試劑: 1、450±10nm濾光片的酶標儀(建議儀器使用前提前預熱);
2、單道或多道微量加液器及吸頭;
3、稀釋樣品的EP管;
4、蒸餾水或去離子水;
5、吸水紙;
6、盛放洗液的容器
Research_Areas: Signal transduction;
Emit: ATPase, H+ Transporting, Lysosomal Accessory Protein 2
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