產品展示首頁 > USCN & cloud-clone 試劑盒 & 抗體 & 蛋白 > cloud-clone 試劑盒、抗體、蛋白 > Visfatin (VF)
內脂素(VF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
貨 號：
SEA638Mu-96T
規(guī) 格：
96T
品 牌：
cloud-clone
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| 英文名稱： | ELISA Kit for Visfatin (VF) |
| 基因別稱： | Nampt; NAmPRTase; PBEF; PBEF1; Pre-B-Cell Colony Enhancing Factor; Nicotinamide Phosphoribosyltransferase |
| 實驗步驟： | 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37oC; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC; 7. Add 50µL Stop Solution. Read at 450 nm immediately. |
| 檢測范圍： | 31.25-2000pg/mL The standard curve concentrations used for the ELISA’s were 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL |
| 最低檢測限： | The minimum detectable dose of this kit is typically less than 13.4pg/mL |
| 適應生物： | Mus musculus (Mouse ，小鼠) |
| 樣本： | Serum, plasma and other biological fluids. |
| 實驗時長： | 3hours |
| 種類： | 參考價格：2721.6/48T ; 3888/96T ; 17496/96T*5 ; 33048/96T*10 ; 272160/96T*100 ; |
| 特異性： | This assay has high sensitivity and excellent specificity for detection of Visfatin (VF). No significant cross-reactivity or interference between Visfatin (VF) and analogues was observed. |
| 精度： | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Visfatin (VF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Visfatin (VF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% |
| 穩(wěn)定性： | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. |
| 實驗原理： | This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Estrone (E1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Estrone (E1) and unlabeled Estrone (E1) (Standards or samples) with the pre-coated antibody specific to Estrone (E1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Estrone (E1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Estrone (E1) in the sample. |
| 質量控制： | ISO13485:2003 EN ISO13485:2012/ISO9001:2008 |
| 產品應用： | ELISA , 酶聯(lián)免疫 |
| 現(xiàn)貨： | 5-7個工作日 |
| 運輸條件： | 藍冰運輸 |
| 保存條件： | 4度和-20度分開保存，避免反復凍融 |
| 有效期： | 6個月 |
| 限制： | 僅供科研使用 |
| 標本量： | 詳見說明書 |
| 需自備的設備及試劑： | 1、450±10nm濾光片的酶標儀(建議儀器使用前提前預熱); 2、單道或多道微量加液器及吸頭; 3、稀釋樣品的EP管; 4、蒸餾水或去離子水; 5、吸水紙; 6、盛放洗液的容器 |
| Research_Areas： | Metabolic pathway;Cardiovascular biology;Hepatology; |
| Emit： | Visfatin |
