產(chǎn)品展示首頁 > USCN & cloud-clone 試劑盒 & 抗體 & 蛋白 > cloud-clone 試劑盒、抗體、蛋白 > Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz)
酪氨酸3/色氨酸5單加氧酶激活蛋白ζ(YWHAz)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
貨 號：
SEJ809Mi-96T
規(guī) 格：
96T
品 牌：
cloud-clone
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| 英文名稱： | ELISA Kit for Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) |
| 基因別稱： | KCIP-1; 14-3-3-zeta; 14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1 |
| 實驗步驟： | 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37oC; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC; 5. Aspirate and wash 5 times; 6. Add 100µL Substrate Solution. Incubate 10 minutes at 37oC; 7. Read RLU value immediately. |
| 檢測范圍： | 0.312-20ng/mL |
| 最低檢測限： | The minimum detectable dose of this kit is typically less than 0.116ng/mL |
| 適應(yīng)生物： | Homo sapiens (Human , 人),Mus musculus (Mouse ，小鼠),Rattus norvegicus (Rat ，大鼠) |
| 樣本： | Tissue homogenates, cell lysates and other biological fluids. |
| 實驗時長： | 3hours |
| 種類： | 參考價格：3024/48T ; 4320/96T ; 19440/96T*5 ; 36720/96T*10 ; 302400/96T*100 ; |
| 特異性： | This assay has high sensitivity and excellent specificity for detection of Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz). No significant cross-reactivity or interference between Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) and analogues was observed. |
| 精度： | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% |
| 穩(wěn)定性： | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. |
| 實驗原理： | The microtiter plate provided in this kit has been pre-coated with a monoclonal antigen. A competitive inhibition reaction is launched between biotin labeled Substance P (SP) and unlabeled Substance P (SP) (Standards or samples) with the pre-coated secondary antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Substance P (SP) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Substance P (SP) level in the sample or standard. |
| 質(zhì)量控制： | ISO13485:2003 EN ISO13485:2012/ISO9001:2008 |
| 產(chǎn)品應(yīng)用： | ELISA , 酶聯(lián)免疫 |
| 現(xiàn)貨： | 5-7個工作日 |
| 運輸條件： | 藍(lán)冰運輸 |
| 保存條件： | 4度和-20度分開保存，避免反復(fù)凍融 |
| 有效期： | 6個月 |
| 限制： | 僅供科研使用 |
| 標(biāo)本量： | 詳見說明書 |
| 需自備的設(shè)備及試劑： | 1、450±10nm濾光片的酶標(biāo)儀(建議儀器使用前提前預(yù)熱); 2、單道或多道微量加液器及吸頭; 3、稀釋樣品的EP管; 4、蒸餾水或去離子水; 5、吸水紙; 6、盛放洗液的容器 |
| Research_Areas： | Enzyme & Kinase; |
