產(chǎn)品展示首頁(yè) > USCN & cloud-clone 試劑盒 & 抗體 & 蛋白 > cloud-clone 試劑盒、抗體、蛋白 > 4-Hydroxyphenylpyruvate Dioxygenase (HPD)
4-羥基苯丙酮酸雙加氧酶(HPD)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
貨 號(hào)：
SEE251Ra-96T
規(guī) 格：
96T
品 牌：
cloud-clone
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| 英文名稱： | ELISA Kit for 4-Hydroxyphenylpyruvate Dioxygenase (HPD) |
| 基因別稱： | PPD; 4-HPPD; 4HPPD; GLOD3; Glyoxalase Domain Containing 3; 4-hydroxyphenylpyruvic acid oxidase |
| 實(shí)驗(yàn)步驟： | 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37oC; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC; 7. Add 50µL Stop Solution. Read at 450 nm immediately. |
| 檢測(cè)范圍： | 3.12-200ng/mL |
| 最低檢測(cè)限： | The minimum detectable dose of this kit is typically less than 1.35ng/mL |
| 適應(yīng)生物： | Rattus norvegicus (Rat ，大鼠) |
| 樣本： | Tissue homogenates, cell lysates and other biological fluids. |
| 實(shí)驗(yàn)時(shí)長(zhǎng)： | 3hours |
| 種類： | 參考價(jià)格：3192/48T ; 4560/96T ; 20520/96T*5 ; 38760/96T*10 ; 319200/96T*100 ; |
| 特異性： | This assay has high sensitivity and excellent specificity for detection of 4-Hydroxyphenylpyruvate Dioxygenase (HPD). No significant cross-reactivity or interference between 4-Hydroxyphenylpyruvate Dioxygenase (HPD) and analogues was observed. |
| 精度： | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 4-Hydroxyphenylpyruvate Dioxygenase (HPD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 4-Hydroxyphenylpyruvate Dioxygenase (HPD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% |
| 穩(wěn)定性： | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. |
| 實(shí)驗(yàn)原理： | This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Leukotriene C4 (LTC4) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Leukotriene C4 (LTC4) and unlabeled Leukotriene C4 (LTC4) (Standards or samples) with the pre-coated antibody specific to Leukotriene C4 (LTC4). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Leukotriene C4 (LTC4) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Leukotriene C4 (LTC4) in the sample. |
| 質(zhì)量控制： | ISO13485:2003 EN ISO13485:2012/ISO9001:2008 |
| 產(chǎn)品應(yīng)用： | ELISA , 酶聯(lián)免疫 |
| 現(xiàn)貨： | 5-7個(gè)工作日 |
| 運(yùn)輸條件： | 藍(lán)冰運(yùn)輸 |
| 保存條件： | 4度和-20度分開(kāi)保存，避免反復(fù)凍融 |
| 有效期： | 6個(gè)月 |
| 限制： | 僅供科研使用 |
| 標(biāo)本量： | 詳見(jiàn)說(shuō)明書(shū) |
| 需自備的設(shè)備及試劑： | 1、450±10nm濾光片的酶標(biāo)儀(建議儀器使用前提前預(yù)熱); 2、單道或多道微量加液器及吸頭; 3、稀釋樣品的EP管; 4、蒸餾水或去離子水; 5、吸水紙; 6、盛放洗液的容器 |
| Research_Areas： | Enzyme & Kinase; |
| Emit： | 4-Hydroxyphenylpyruvate Dioxygenase |
