產(chǎn)品展示首頁 > USCN & cloud-clone 試劑盒 & 抗體 & 蛋白 > cloud-clone 試劑盒、抗體、蛋白 > Autophagy Related Protein 5 (ATG5)
自噬相關(guān)蛋白5(ATG5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
貨 號：
SEL221Hu-96T
規(guī) 格：
96T
品 牌：
cloud-clone
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| 英文名稱： | ELISA Kit for Autophagy Related Protein 5 (ATG5) |
| 基因別稱： | ASP; APG5; APG5L; hAPG5; Apoptosis-specific protein; Autophagy protein 5 |
| 實驗步驟： | 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37oC; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC; 7. Add 50µL Stop Solution. Read at 450 nm immediately. |
| 檢測范圍： | 0.78-50ng/mL |
| 最低檢測限： | The minimum detectable dose of this kit is typically less than 0.28ng/mL |
| 適應(yīng)生物： | Homo sapiens (Human , 人) |
| 樣本： | Serum, plasma, tissue homogenates and other biological fluids |
| 實驗時長： | 3hours |
| 種類： | 參考價格：3234/48T ; 4620/96T ; 20790/96T*5 ; 39270/96T*10 ; 323400/96T*100 ; |
| 特異性： | This assay has high sensitivity and excellent specificity for detection of Autophagy Related Protein 5 (ATG5). No significant cross-reactivity or interference between Autophagy Related Protein 5 (ATG5) and analogues was observed. |
| 精度： | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Autophagy Related Protein 5 (ATG5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Autophagy Related Protein 5 (ATG5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% |
| 穩(wěn)定性： | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. |
| 實驗原理： | This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Glucagon Like Peptide 2 (GLP2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Glucagon Like Peptide 2 (GLP2) and unlabeled Glucagon Like Peptide 2 (GLP2) (Standards or samples) with the pre-coated antibody specific to Glucagon Like Peptide 2 (GLP2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Glucagon Like Peptide 2 (GLP2) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Glucagon Like Peptide 2 (GLP2) in the sample. |
| 質(zhì)量控制： | ISO13485:2003 EN ISO13485:2012/ISO9001:2008 |
| 產(chǎn)品應(yīng)用： | ELISA , 酶聯(lián)免疫 |
| 現(xiàn)貨： | 5-7個工作日 |
| 運輸條件： | 藍(lán)冰運輸 |
| 保存條件： | 4度和-20度分開保存，避免反復(fù)凍融 |
| 有效期： | 6個月 |
| 限制： | 僅供科研使用 |
| 標(biāo)本量： | 詳見說明書 |
| 需自備的設(shè)備及試劑： | 1、450±10nm濾光片的酶標(biāo)儀(建議儀器使用前提前預(yù)熱); 2、單道或多道微量加液器及吸頭; 3、稀釋樣品的EP管; 4、蒸餾水或去離子水; 5、吸水紙; 6、盛放洗液的容器 |
| Research_Areas： | Metabolic pathway; |
| Emit： | Autophagy Related Protein 5 |
