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產(chǎn)品展示首頁 > USCN & cloud-clone 試劑盒 & 抗體 & 蛋白 > USCN & ELISA試劑盒 > 酶聯(lián)免疫試劑盒/elisa試劑盒(小樣本型）

膽囊收縮素八肽(CCK8)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)

貨號：

USCN-MEB044Hu

規(guī) 格：

品牌：

Uscn

產(chǎn)品描述
參考文獻
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說明書下載

英文名稱：

Mini Samples ELISA Kit for Cholecystokinin 8 (CCK8)

基因別稱：

Sincalide; Cholecystokinin 8, Octapeptide

實驗步驟：

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well.
And then add 25μL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 50µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 50µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 25µL Stop Solution. Read at 450 nm immediately.

檢測范圍：

2.47-200pg/mL

最低檢測限：

The minimum detectable dose of this kit is typically less than 0.87pg/mL

適應(yīng)生物：

Homo sapiens (Human)

樣本：

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

種類：

Competitive Inhibition

特異性：

This assay has high sensitivity and excellent specificity for detection of Mini Samples Cholecystokinin 8 (CCK8).
No significant cross-reactivity or interference between Mini Samples Cholecystokinin 8 (CCK8) and analogues was observed.

精度：

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Cholecystokinin 8 (CCK8) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Cholecystokinin 8 (CCK8) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

穩(wěn)定性：

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

實驗原理：

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Cholecystokinin 8 (CCK8) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Cholecystokinin 8 (CCK8) and unlabeled Mini Samples Cholecystokinin 8 (CCK8) (Standards or samples) with the pre-coated antibody specific to Mini Samples Cholecystokinin 8 (CCK8). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Cholecystokinin 8 (CCK8) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Cholecystokinin 8 (CCK8) in the sample.

產(chǎn)品應(yīng)用：

Enzyme-linked immunosorbent assay for Antigen Detection.

應(yīng)用備注：

48T, 96T, 96T×5, 96T×10, 96T×100

期貨：

5-7天

運輸條件：

藍冰運輸

保存條件：

按要求4度和-20度分開保存

有效期：

12個月

限制：

僅用科研 ( ONLY USE R&D research )

需自備的設(shè)備及試劑：

450nm酶標儀 & 移液器等

Research_Areas：

Endocrinology;Gastroenterology;Hormone metabolism;

Emit：

Cholecystokinin 8

Excite：

一、ELISA試劑盒代測（凡購買本公司的產(chǎn)品）

1. 提供代測產(chǎn)品的貨號，英文全名及縮寫；

2. 提供要檢測的樣本信息（比如：樣本類型，數(shù)量，保存條件，保存時間等）；

3. 提供聯(lián)系人的全名，單位，電話等信息；

4. 簽訂代測協(xié)議；

5. 樣本需干冰運輸，收到標本后一周內(nèi)安排檢測實驗，十個工作日左右出具檢測報告。

二、免疫組化實驗（免費）

購買本公司的產(chǎn)品。

三、售前、售中、售后的技術(shù)支持（隨時）

說明書下載

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