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產(chǎn)品展示首頁(yè) > USCN & cloud-clone 試劑盒 & 抗體 & 蛋白 > USCN & ELISA試劑盒 > 酶聯(lián)免疫試劑盒/elisa試劑盒(檢測(cè)抗體型)
抗替度魯肽抗體(Anti-TFA)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
貨    號(hào):
USCN-AEX304Ge
規(guī)    格:
品    牌:
Uscn
英文名稱: ELISA Kit for Anti-Teduglutide Antibody (Anti-TFA)
實(shí)驗(yàn)步驟: 1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 5 times;
5. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
6. Add 50µL Stop Solution. Read at 450nm immediately.
檢測(cè)范圍: 3.12-200ng/mL
最低檢測(cè)限: The minimum detectable dose of this kit is typically less than 1.22ng/mL
適應(yīng)生物: Pan-species (General)
樣本: biological agents
實(shí)驗(yàn)時(shí)長(zhǎng): 2h, 30min
種類: Competitive Inhibition
特異性:

This assay has high sensitivity and excellent specificity for detection of Anti-Teduglutide Antibody (Anti-TFA).
No significant cross-reactivity or interference between Anti-Teduglutide Antibody (Anti-TFA) and analogues was observed.

精度:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Teduglutide Antibody (Anti-TFA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Teduglutide Antibody (Anti-TFA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

穩(wěn)定性:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

實(shí)驗(yàn)原理: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Teduglutide Antibody (Anti-TFA) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Teduglutide Antibody (Anti-TFA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
產(chǎn)品應(yīng)用: Enzyme-linked immunosorbent assay for Antibody Detection.
應(yīng)用備注: 48T, 96T, 96T×5, 96T×10, 96T×100
期貨: 5-7天
運(yùn)輸條件: 藍(lán)冰運(yùn)輸
保存條件: 按要求4度和-20度分開(kāi)保存
有效期: 12個(gè)月
限制: 僅用科研 ( ONLY USE R&D research )
需自備的設(shè)備及試劑: 450nm酶標(biāo)儀 & 移液器等
Research_Areas: Endocrinology;Gastroenterology;Hormone metabolism;
Emit: Teduglutide
Excite: -
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