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英文名稱: | ELISA Kit for Anti-Myosin Heavy Chain 7B, Cardiac Muscle Beta Antibody (Anti-MYH7B) |
基因別稱: | MYH14; MHC14; Antigen MLAA-21; Myosin cardiac muscle beta chain |
實(shí)驗(yàn)步驟: | 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 5 times; 5. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 6. Add 50µL Stop Solution. Read at 450nm immediately. |
檢測(cè)范圍: | 3.12-200ng/mL |
最低檢測(cè)限: | The minimum detectable dose of this kit is typically less than 1.25ng/mL |
適應(yīng)生物: | Homo sapiens (Human) |
樣本: | serum, plasma and other biological fluids |
實(shí)驗(yàn)時(shí)長(zhǎng): | 2h, 30min |
種類: | Competitive Inhibition |
特異性: |
This assay has high sensitivity and excellent specificity for detection of Anti-Myosin Heavy Chain 7B, Cardiac Muscle Beta Antibody (Anti-MYH7B). |
精度: |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Myosin Heavy Chain 7B, Cardiac Muscle Beta Antibody (Anti-MYH7B) were tested 20 times on one plate, respectively. |
穩(wěn)定性: |
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
實(shí)驗(yàn)原理: | The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Myosin Heavy Chain 7B, Cardiac Muscle Beta Antibody (Anti-MYH7B) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Myosin Heavy Chain 7B, Cardiac Muscle Beta Antibody (Anti-MYH7B) in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
產(chǎn)品應(yīng)用: | Enzyme-linked immunosorbent assay for Antibody Detection. |
應(yīng)用備注: | 48T, 96T, 96T×5, 96T×10, 96T×100 |
期貨: | 5-7天 |
運(yùn)輸條件: | 藍(lán)冰運(yùn)輸 |
保存條件: | 按要求4度和-20度分開(kāi)保存 |
有效期: | 12個(gè)月 |
限制: | 僅用科研 ( ONLY USE R&D research ) |
需自備的設(shè)備及試劑: | 450nm酶標(biāo)儀 & 移液器等 |
Research_Areas: | Cardiovascular biology; |
Emit: | Myosin Heavy Chain 7B, Cardiac Muscle Beta |
Excite: | A7E2Y1 |