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產(chǎn)品展示首頁 > USCN & cloud-clone 試劑盒 & 抗體 & 蛋白 > USCN & ELISA試劑盒 > 酶聯(lián)免疫試劑盒/elisa試劑盒(競爭法)
肝素(Hep)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
貨    號(hào):
USCN-CEV639Ge
規(guī)    格:
96wells
品    牌:
Uscn
英文名稱: ELISA Kit for Heparin (Hep)
基因別稱: Unfractionated heparin
實(shí)驗(yàn)步驟: 1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
檢測(cè)范圍: 12.35-1,000ng/mL
最低檢測(cè)限: The minimum detectable dose of this kit is typically less than 4.33ng/mL
適應(yīng)生物: Pan-species (General)
樣本: serum, plasma and other biological fluids
實(shí)驗(yàn)時(shí)長: 2h
種類: Competitive Inhibition
特異性:

This assay has high sensitivity and excellent specificity for detection of Heparin (Hep).
No significant cross-reactivity or interference between Heparin (Hep) and analogues was observed.

精度:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heparin (Hep) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heparin (Hep) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

穩(wěn)定性:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

實(shí)驗(yàn)原理: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Heparin (Hep) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Heparin (Hep) and unlabeled Heparin (Hep) (Standards or samples) with the pre-coated antibody specific to Heparin (Hep). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Heparin (Hep) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Heparin (Hep) in the sample.
產(chǎn)品應(yīng)用: Enzyme-linked immunosorbent assay for Antigen Detection.
應(yīng)用備注: 48T, 96T, 96T×5, 96T×10, 96T×100
期貨: 5-7天
運(yùn)輸條件: 藍(lán)冰運(yùn)輸
保存條件: 按要求4度和-20度分開保存
有效期: 12個(gè)月
限制: 僅用科研 ( ONLY USE R&D research )
Comment: 需要代測(cè)請(qǐng)聯(lián)系說明
需自備的設(shè)備及試劑: 450nm酶標(biāo)儀 & 移液器等
Emit: Heparin
Excite: -
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