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英文名稱: | ELISA Kit for Immunoglobulin G (IgG) |
實(shí)驗(yàn)步驟: | 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 5 times; 4. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 5. Add 50µL Stop Solution. Read at 450 nm immediately. |
檢測范圍: | 1.23-100ug/mL |
最低檢測限: | The minimum detectable dose of this kit is typically less than 0.53ug/mL |
適應(yīng)生物: | Bos taurus; Bovine (Cattle) |
樣本: | Serum, plasma and other biological fluids |
實(shí)驗(yàn)時(shí)長: | 2h |
種類: | Competitive Inhibition |
特異性: |
This assay has high sensitivity and excellent specificity for detection of Immunoglobulin G (IgG). |
精度: |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Immunoglobulin G (IgG) were tested 20 times on one plate, respectively. |
穩(wěn)定性: |
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
實(shí)驗(yàn)原理: | This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to Immunoglobulin G (IgG) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled Immunoglobulin G (IgG) and unlabeled Immunoglobulin G (IgG) (Standards or samples) with the pre-coated antibody specific to Immunoglobulin G (IgG). After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of Immunoglobulin G (IgG) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Immunoglobulin G (IgG) in the sample. |
產(chǎn)品應(yīng)用: | Enzyme-linked immunosorbent assay for Antigen Detection. |
應(yīng)用備注: | 48T, 96T, 96T×5, 96T×10, 96T×100 |
期貨: | 5-7天 |
運(yùn)輸條件: | 藍(lán)冰運(yùn)輸 |
保存條件: | 按要求4度和-20度分開保存 |
有效期: | 12個(gè)月 |
限制: | 僅用科研 ( ONLY USE R&D research ) |
Comment: | 需要代測請聯(lián)系說明 |
需自備的設(shè)備及試劑: | 450nm酶標(biāo)儀 & 移液器等 |
Research_Areas: | Infection immunity;Immune molecule;Hematology; |
Emit: | Immunoglobulin G |
Excite: | - |