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產(chǎn)品展示首頁 > USCN & cloud-clone 試劑盒 & 抗體 & 蛋白 > USCN & ELISA試劑盒 > 酶聯(lián)免疫試劑盒/elisa試劑盒(夾心法）

泛素特異性肽酶14(USP14)檢測試劑盒(酶聯(lián)免疫吸附試驗法)

貨號：

USCN-SEF134Hu

規(guī) 格：

96wells

品牌：

Uscn

產(chǎn)品描述
參考文獻
相關產(chǎn)品
售后保障
說明書下載

英文名稱：

ELISA Kit for Ubiquitin Specific Peptidase 14 (USP14)

基因別稱：

TGT; tRNA-Guanine Transglycosylase; Ubiquitin Specific Protease 14; Deubiquitinating enzyme 14; Ubiquitin carboxyl-terminal hydrolase 14

實驗步驟：

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

檢測范圍：

0.156-10ng/mL

最低檢測限：

The minimum detectable dose of this kit is typically less than 0.057ng/mL

適應生物：

Homo sapiens (Human)

樣本：

tissue homogenates, cell lysates and other biological fluids

實驗時長：

種類：

Double-antibody Sandwich

特異性：

This assay has high sensitivity and excellent specificity for detection of Ubiquitin Specific Peptidase 14 (USP14).
No significant cross-reactivity or interference between Ubiquitin Specific Peptidase 14 (USP14) and analogues was observed.

精度：

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin Specific Peptidase 14 (USP14) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin Specific Peptidase 14 (USP14) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

穩(wěn)定性：

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

實驗原理：

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Specific Peptidase 14 (USP14). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin Specific Peptidase 14 (USP14). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin Specific Peptidase 14 (USP14), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ubiquitin Specific Peptidase 14 (USP14) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

產(chǎn)品應用：

Enzyme-linked immunosorbent assay for Antigen Detection.

應用備注：

48T, 96T, 96T×5, 96T×10, 96T×100

期貨：

5-7天

運輸條件：

藍冰運輸

保存條件：

按要求4度和-20度分開保存

有效期：

12個月

限制：

僅用科研 ( ONLY USE R&D research )

需自備的設備及試劑：

450nm酶標儀 & 移液器等

Research_Areas：

Enzyme & Kinase;

Emit：

Ubiquitin Specific Peptidase 14

Excite：

P54578

一、ELISA試劑盒代測（凡購買本公司的產(chǎn)品）

1. 提供代測產(chǎn)品的貨號，英文全名及縮寫；

2. 提供要檢測的樣本信息（比如：樣本類型，數(shù)量，保存條件，保存時間等）；

3. 提供聯(lián)系人的全名，單位，電話等信息；

4. 簽訂代測協(xié)議；

5. 樣本需干冰運輸，收到標本后一周內(nèi)安排檢測實驗，十個工作日左右出具檢測報告。

二、免疫組化實驗（免費）

購買本公司的產(chǎn)品。

三、售前、售中、售后的技術支持（隨時）

說明書下載

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